Motivation

Specific leaf area (SLA, the ratio of leaf area to leaf dry mass) is an important trait for plant physiological, structural, and modeling analyses. SLA is also a useful measurement to upscale plant production, vegetation community composition, and other processes in Earth System Models, providing current-day benchmarks and improving predictions of future vegetation structure and function in terrestrial ecosystems.

We investigate SLA differences between plots of varying elevation and salinity exposure along a tributary of the Chesapeake Bay. The goals of this project are to (i) quantify SLA of eight major tree species, at multiple canopy heights, in temperate deciduous forests at the Smithsonian Environmental Research Center (Maryland, USA); and (ii) test for effects of soil moisture and saltwater exposure on SLA by comparing samples taken at shoreline and upland positions, with the shoreline samples taken along a ~2 km tidal creek that provided a natural salinity gradient.

Map of SERC with plots labeled:

Sampling Design

I think this would be a good place for methods

Figure 1: Collecting samples using a pole pruner

Figure 1: Collecting samples using a pole pruner

Figure 2: Collecting samples using a hand pruner

Figure 2: Collecting samples using a hand pruner

Figure 3: All the samples after a morning of collecting

Figure 3: All the samples after a morning of collecting

SLA Calculation

SLA is calculated using the ratio between a leaf’s one-sided fresh leaf area and its total dry mass.

sla <- sla %>% 
  mutate(specific_leaf_area = round(Leaf_Area_cm2 / Leaf_Mass_g, 3)) 

Average Specific Leaf Area

Specific Leaf Area by Plot

Specific Leaf Area by Species

The Relationship Between Specific Leaf Area and Tree Diameter at Breast Height